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FIGURE 8 | The schematic diagram of the regulatory mechanism illustrates that PDLIM5 mediates mechanical stimulation, thereby promoting osteogenic differentiation of human adipose-derived stem cells (hASCs) via the serpin E2/integrin β3-PDLIM5-lamin A signalling axis.

Journal: Cell proliferation

Article Title: Mechanotransduction Mediated by PDLIM5: The Critical Role of Serpin E2/Integrin β3-Cytoskeleton-Nucleoskeleton Axis in Mechanical Osteogenic Programming.

doi: 10.1111/cpr.70067

Figure Lengend Snippet: FIGURE 8 | The schematic diagram of the regulatory mechanism illustrates that PDLIM5 mediates mechanical stimulation, thereby promoting osteogenic differentiation of human adipose-derived stem cells (hASCs) via the serpin E2/integrin β3-PDLIM5-lamin A signalling axis.

Article Snippet: The blots were then incubated overnight at 4°C with the following primary antibodies: anti- Serpin E2 antibody (ab154591, Abcam, Cambridge, UK, dilution 1:1500), anti- Integrin β3 antibody (orb214124, biorbyt, dilution 1:1000), anti- PDLIM5 antibody (ab85967, dilution 1:1500; 10530- 1- AP, proteintech, USA, dilution 1:1000), anti- α- actinin 1 antibody (ab68194, dilution 1:1500), anti- lamin A antibody (ab8984, dilution 1:1500; 10298- 1- AP, proteintech, USA, dilution 1:1000), anti- osteopontin (OPN) antibody (ab69498, dilution 1:1500), anti- RUNT- related transcription factor 2 (RUNX2) antibody (CST#12556S, Cell Signalling Technology, Danvers, MA, dilution 1:1000) and anti- GAPDH antibody (AP0063, dilution 1:5000, Bioworld, Bloomington, MN).

Techniques: Derivative Assay